Britain's Nicole Cooke leads the Emakumeen Bira event in Spain after winning Friday's second stage.
Olympic road race champion Cooke, 26, triumphed on an 84km loop, which started and finished in Amurrio, with an uphill surge to the line.
The Welsh rider's Vision 1 Racing team also lead the team competition.
"I am really pleased with how we are riding as a team. The girls are working really hard for me," said Cooke, who came second in the opening stage.
Stage three runs on Saturday in two parts, a 2.2km uphill time trial from Iurreta to Goiuria and a 79km loop out of Elgoibar.